Virus isolation is available for a range of pathogens, including salmonid alphavirus
(SAV).
Immunofluorescent staining
of infectious pancreatic necrosis virus growing in cell culture
- SAV isolation
Isolation of SAV (SPDV, SDV) has traditionally
been carried out from tissue samples.
- This is labour intensive and limits the number of samples that can be processed.
- Tissues are typically collected when clinical signs are evident: this can be several weeks
after initial exposure, reducing the likelihood of virus still being present.
- It is now recognised that following infection, Atlantic salmon and trout have a period
of viraemia (up to two weeks) and that serum samples provide an excellent analyte for virus isolation
Jewhurst
et al. 2004; McLoughlin et al. 2006; Graham et al. 2007.
When
combined with immunostaining to reveal the presence of virus, positive results can be obtained in as
little as three days.
- This test is typically conducted in conjunction with serology testing for
virus neutralizing antibodies.
- Virus isolation.
- Serum should be separated and sent on ice. Avoid freezing if possible.
- Tissues should be sent on ice. Avoid freezing if possible. Ideally, samples should be
in transport medium, as outlined in Packaging of Samples. Packaging of samples.
For more information contact:
Dr Michael Welsh
Agri-Food
& Biosciences Institute (AFBI)
Virology Branch
Veterinary Sciences
Division
Stoney Road
Stormont
Belfast BT4 3SD
U.K.
Email
Dr Michael WelshTel +44 (0) 28905 25672
Fax +44 (0)
2890525773
Work
Mobile +44 (0)7900212807
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