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Specific research questions

B) Economic potential of the predatory mite Hypoaspis miles to suppress Sciaridae in mushroom cultures

B1) Dose response of H. miles to suppress Sciaridae

Method:

Containers of 2 litre volume with 300g of spawned compost exposed to adult Sciaridae to lay eggs.

Treatments:

  • Predatory mites at several doses
  • Diflubenzuron (Dimilin) at a commercial rate (1g AI/m2)
  • Untreated control
  • Catch emerging flies with sticky cards on the lids of the container.

Results:

At approximately 400 adult mites / m2, H. miles provides similar sciarid suppression as diflubenzuron treatment.



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B2) Preliminary comparison of mites, nematodes and insecticides in an Irish bag system

Method:

Experiment conducted with a crop grown in 20 kg compost bags.

Mites were applied in a “non-commercial” manner (individual mites counted out from mite culture substrate).

Treatments applied:

  1. Untreated control
  2. Insecticide check (diazinon granules at 25mg AI/kg compost, diflubenzuron at 1g AI/m2 drench into casing
  3. 710 H. miles/m2 applied at begin spawn run
  4. 710 H. miles/m2 applied at begin case run
  5. 3 x 106 S. feltiae/m2 applied at begin spawn run
  6. 3 x 106 S. feltiae/m2 applied at begin case run

Results:

H. miles (710 mites/m2) applied at beginning of spawn run, suppresses L. ingenua until mid-case run. Later application of H. miles exhibited no effect. S. feltia (3x106/m2), applied at beginning of spawn run had no effect. However, when applied at the beginning of case run, L. ingenua suppression was evident until the the 3rd flush.



Neither H. miles nor S. feltiae exhibited Any negative effects on mushroom yield.


Conclusion:

At present costs, and with a dose response function established under laboratory conditions, predatory mites (H. miles) and insecticides may provide a similar cost-benefit ratio in suppressing Sciaridae.
Sciarid suppression by H. miles in a mushroom crop (Irish bag system) has been demonstrated. When H. miles was applied without its carrier material (vermiculite, peat, and prey) no negative effect on the crop yield was noticed.
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